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Method for the Detection of Pepino mosaic virus on Tomato seed

1.1 Grind seeds of each sub-sample in a 10 ml seed extraction buffer. Process extracts immediately after grinding or store at 4°C for a maximum of 20 hours.

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Tomato – Pepino mosaic virus. Method for the Detection of Pepino mosaic virus on Tomato seed. Crop Tomato Grind seeds of each sub sample in a 10 ml seed extraction buffer. Process Clark M.F. and A.N. Adams 1977 Characteristics of the microplate …

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Method for the Detection of Pepino mosaic virus on Tomato seed

Extraction of virus from the seed . 1.1 Grind seeds of each sub-sample in a 10 ml ... Characteristics of the microplate method of enzyme-linked immunosorbent assay ...

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Grinder: capable of grinding seeds to fine flour Micropipette Micropipette tips Microplate spectrophotometer capable of operation at 405 nm. PROCEDURE: Preparation of ELISA plate: 1. Dilute Pantoea stewartii coating antibody in coating buffer as defined by the supplier. 2. Add 100 μl of coating solution to each well. 3. Cover …

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Mz 11.1 Maize Dwarf Mosaic Virus - NSHS – …

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